Wolves winger Daniel Podence has denied spitting at Nottingham Forest's Brennan Johnson, saying he would "never do such a thing".
Podence was charged by the Football Association on Wednesday after Johnson alleged Podence had spat at him in the 90th minute of Saturday's 1-1 draw.
Referee Chris Kavanagh took no action after a video assistant referee checked at the City Ground.
Podence, 27, said he had been "accused of something I haven't done".
Writing on Instagram, he added: "I will make it clear: I did not spit on Nottingham's player. I'm telling the truth and I would never do such a thing, even more to a colleague."
Portuguese Podence, who scored Wolves' late equaliser in the Premier League game against Forest, has until 12 April to respond to the FA's charge.
The standard punishment for spitting is a six-match suspension.
Speaking at a news conference on Thursday, Wolves boss Julen Lopetegui said Podence "never, ever spat at someone, that's 100%", before adding the club is "working on it".
Podence has scored six goals in the league this season. Wolves host Chelsea on Saturday (15:00 BST).